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lfb optical density analysis approach  (Nikon)


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    Nikon lfb optical density analysis approach
    Histology and ROI sampling. Left, top: one of the four post-mortem brain samples with the corpus callosum in situ. Left, bottom: template of the corpus callosum showing 92 circular regions of interest (ROIs) based on the anatomical subdivision proposed by Witelson . Values were calculated inside the circular ROIs in the centre of each of the 92 squares of the callosal template and then extended to the square surrounding the circle. Centre: <t>Luxol</t> <t>Fast</t> <t>Blue</t> <t>(LFB)-stained</t> midsagittal sections (25 µm thick) of the corpora callosa of subjects 1, 2 and 3. Right: ROI-based, colour-coded maps of the myelin distribution of subjects 1, 2 and 3 along the midsagittal section of the corpus callosum assessed ex vivo via histological myelin staining. The histological myelin distribution pattern of the corpus callosum is different from the T1w/T2w pattern. All three LFB callosal samples have low myelin density in the anterior part (mainly rostrum/genu) and higher myelin density in the posterior part (mainly isthmus/splenium). Colour scale: purple, low values; red, high values
    Lfb Optical Density Analysis Approach, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 37545 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lfb optical density analysis approach/product/Nikon
    Average 99 stars, based on 37545 article reviews
    lfb optical density analysis approach - by Bioz Stars, 2026-05
    99/100 stars

    Images

    1) Product Images from "Mapping myelin in white matter with T1-weighted/T2-weighted maps: discrepancy with histology and other myelin MRI measures"

    Article Title: Mapping myelin in white matter with T1-weighted/T2-weighted maps: discrepancy with histology and other myelin MRI measures

    Journal: Brain Structure & Function

    doi: 10.1007/s00429-022-02600-z

    Histology and ROI sampling. Left, top: one of the four post-mortem brain samples with the corpus callosum in situ. Left, bottom: template of the corpus callosum showing 92 circular regions of interest (ROIs) based on the anatomical subdivision proposed by Witelson . Values were calculated inside the circular ROIs in the centre of each of the 92 squares of the callosal template and then extended to the square surrounding the circle. Centre: Luxol Fast Blue (LFB)-stained midsagittal sections (25 µm thick) of the corpora callosa of subjects 1, 2 and 3. Right: ROI-based, colour-coded maps of the myelin distribution of subjects 1, 2 and 3 along the midsagittal section of the corpus callosum assessed ex vivo via histological myelin staining. The histological myelin distribution pattern of the corpus callosum is different from the T1w/T2w pattern. All three LFB callosal samples have low myelin density in the anterior part (mainly rostrum/genu) and higher myelin density in the posterior part (mainly isthmus/splenium). Colour scale: purple, low values; red, high values
    Figure Legend Snippet: Histology and ROI sampling. Left, top: one of the four post-mortem brain samples with the corpus callosum in situ. Left, bottom: template of the corpus callosum showing 92 circular regions of interest (ROIs) based on the anatomical subdivision proposed by Witelson . Values were calculated inside the circular ROIs in the centre of each of the 92 squares of the callosal template and then extended to the square surrounding the circle. Centre: Luxol Fast Blue (LFB)-stained midsagittal sections (25 µm thick) of the corpora callosa of subjects 1, 2 and 3. Right: ROI-based, colour-coded maps of the myelin distribution of subjects 1, 2 and 3 along the midsagittal section of the corpus callosum assessed ex vivo via histological myelin staining. The histological myelin distribution pattern of the corpus callosum is different from the T1w/T2w pattern. All three LFB callosal samples have low myelin density in the anterior part (mainly rostrum/genu) and higher myelin density in the posterior part (mainly isthmus/splenium). Colour scale: purple, low values; red, high values

    Techniques Used: Sampling, In Situ, Staining, Ex Vivo

    To quantitatively evaluate the correlation between T1w/T2w, MWF and histology, MRI maps were sampled using the same 92-ROI sampling scheme used for histology and correlated with the corresponding average LFB-OD values. Data is displayed on arbitrary units (from 1 to 7) for all methods. A T1w/T2w does not positively correlate with the LFB-Optical Density analysis but, instead, has a significant negative correlation ( r 2 = 0.21 p < 0.001). B MWF presents a strong and significant positive correlation with LFB ( r 2 = 0.52, p < 0.001), suggesting a good agreement between the two modalities. C T1w/T2w and MWF maps do not correlate positively, as they are characterised by a weak negative correlation ( r 2 = 0.13, p < 0.001)
    Figure Legend Snippet: To quantitatively evaluate the correlation between T1w/T2w, MWF and histology, MRI maps were sampled using the same 92-ROI sampling scheme used for histology and correlated with the corresponding average LFB-OD values. Data is displayed on arbitrary units (from 1 to 7) for all methods. A T1w/T2w does not positively correlate with the LFB-Optical Density analysis but, instead, has a significant negative correlation ( r 2 = 0.21 p < 0.001). B MWF presents a strong and significant positive correlation with LFB ( r 2 = 0.52, p < 0.001), suggesting a good agreement between the two modalities. C T1w/T2w and MWF maps do not correlate positively, as they are characterised by a weak negative correlation ( r 2 = 0.13, p < 0.001)

    Techniques Used: Sampling



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    lfb optical density analysis approach  (Nikon)
    99
    Nikon lfb optical density analysis approach
    Histology and ROI sampling. Left, top: one of the four post-mortem brain samples with the corpus callosum in situ. Left, bottom: template of the corpus callosum showing 92 circular regions of interest (ROIs) based on the anatomical subdivision proposed by Witelson . Values were calculated inside the circular ROIs in the centre of each of the 92 squares of the callosal template and then extended to the square surrounding the circle. Centre: <t>Luxol</t> <t>Fast</t> <t>Blue</t> <t>(LFB)-stained</t> midsagittal sections (25 µm thick) of the corpora callosa of subjects 1, 2 and 3. Right: ROI-based, colour-coded maps of the myelin distribution of subjects 1, 2 and 3 along the midsagittal section of the corpus callosum assessed ex vivo via histological myelin staining. The histological myelin distribution pattern of the corpus callosum is different from the T1w/T2w pattern. All three LFB callosal samples have low myelin density in the anterior part (mainly rostrum/genu) and higher myelin density in the posterior part (mainly isthmus/splenium). Colour scale: purple, low values; red, high values
    Lfb Optical Density Analysis Approach, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lfb optical density analysis approach/product/Nikon
    Average 99 stars, based on 1 article reviews
    lfb optical density analysis approach - by Bioz Stars, 2026-05
    99/100 stars
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    Image Search Results


    Histology and ROI sampling. Left, top: one of the four post-mortem brain samples with the corpus callosum in situ. Left, bottom: template of the corpus callosum showing 92 circular regions of interest (ROIs) based on the anatomical subdivision proposed by Witelson . Values were calculated inside the circular ROIs in the centre of each of the 92 squares of the callosal template and then extended to the square surrounding the circle. Centre: Luxol Fast Blue (LFB)-stained midsagittal sections (25 µm thick) of the corpora callosa of subjects 1, 2 and 3. Right: ROI-based, colour-coded maps of the myelin distribution of subjects 1, 2 and 3 along the midsagittal section of the corpus callosum assessed ex vivo via histological myelin staining. The histological myelin distribution pattern of the corpus callosum is different from the T1w/T2w pattern. All three LFB callosal samples have low myelin density in the anterior part (mainly rostrum/genu) and higher myelin density in the posterior part (mainly isthmus/splenium). Colour scale: purple, low values; red, high values

    Journal: Brain Structure & Function

    Article Title: Mapping myelin in white matter with T1-weighted/T2-weighted maps: discrepancy with histology and other myelin MRI measures

    doi: 10.1007/s00429-022-02600-z

    Figure Lengend Snippet: Histology and ROI sampling. Left, top: one of the four post-mortem brain samples with the corpus callosum in situ. Left, bottom: template of the corpus callosum showing 92 circular regions of interest (ROIs) based on the anatomical subdivision proposed by Witelson . Values were calculated inside the circular ROIs in the centre of each of the 92 squares of the callosal template and then extended to the square surrounding the circle. Centre: Luxol Fast Blue (LFB)-stained midsagittal sections (25 µm thick) of the corpora callosa of subjects 1, 2 and 3. Right: ROI-based, colour-coded maps of the myelin distribution of subjects 1, 2 and 3 along the midsagittal section of the corpus callosum assessed ex vivo via histological myelin staining. The histological myelin distribution pattern of the corpus callosum is different from the T1w/T2w pattern. All three LFB callosal samples have low myelin density in the anterior part (mainly rostrum/genu) and higher myelin density in the posterior part (mainly isthmus/splenium). Colour scale: purple, low values; red, high values

    Article Snippet: We estimated myelin density using an LFB Optical Density analysis approach (Beckmann et al. ) with the Nikon NIS-Elements Advanced Research 4.12 software.

    Techniques: Sampling, In Situ, Staining, Ex Vivo

    To quantitatively evaluate the correlation between T1w/T2w, MWF and histology, MRI maps were sampled using the same 92-ROI sampling scheme used for histology and correlated with the corresponding average LFB-OD values. Data is displayed on arbitrary units (from 1 to 7) for all methods. A T1w/T2w does not positively correlate with the LFB-Optical Density analysis but, instead, has a significant negative correlation ( r 2 = 0.21 p < 0.001). B MWF presents a strong and significant positive correlation with LFB ( r 2 = 0.52, p < 0.001), suggesting a good agreement between the two modalities. C T1w/T2w and MWF maps do not correlate positively, as they are characterised by a weak negative correlation ( r 2 = 0.13, p < 0.001)

    Journal: Brain Structure & Function

    Article Title: Mapping myelin in white matter with T1-weighted/T2-weighted maps: discrepancy with histology and other myelin MRI measures

    doi: 10.1007/s00429-022-02600-z

    Figure Lengend Snippet: To quantitatively evaluate the correlation between T1w/T2w, MWF and histology, MRI maps were sampled using the same 92-ROI sampling scheme used for histology and correlated with the corresponding average LFB-OD values. Data is displayed on arbitrary units (from 1 to 7) for all methods. A T1w/T2w does not positively correlate with the LFB-Optical Density analysis but, instead, has a significant negative correlation ( r 2 = 0.21 p < 0.001). B MWF presents a strong and significant positive correlation with LFB ( r 2 = 0.52, p < 0.001), suggesting a good agreement between the two modalities. C T1w/T2w and MWF maps do not correlate positively, as they are characterised by a weak negative correlation ( r 2 = 0.13, p < 0.001)

    Article Snippet: We estimated myelin density using an LFB Optical Density analysis approach (Beckmann et al. ) with the Nikon NIS-Elements Advanced Research 4.12 software.

    Techniques: Sampling